Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface
نویسندگان
چکیده
Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta 2-glycoprotein I (beta 2-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C = O moieties. aCL derived from either APS patients or (NZW x BXSB)F1 mice bound to beta 2-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta 2-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta 2-GPI but were unaffected by addition of excess beta 2-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta 2-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta 2-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta 2-GPI. This epitope is expressed by a conformational change occurring when beta 2-GPI interacts with an oxygen-substituted solid phase surface.
منابع مشابه
Revisiting Beta 2 Glycoprotein I, the Major Autoantigen in the Antiphospholipid Syndrome
Beta 2 glycoprotein I (β2GPI) is a single chain 50 kDa highly glycosylated glycoprotein at an approximate concentration of 4 μM in cells. The abundance of this protein in plasma and its high state of preservation indicate the important role of this protein in mammalian. In addition, β2GPI has a particular structure in the fifth domain, and is categorized as the major antigen recognized by autoa...
متن کاملSignificance of anticardiolipin and anti-beta(2)-glycoprotein I antibodies in lupus nephritis.
OBJECTIVE To investigate whether anticardiolipin (aCL) and anti-beta(2)-glycoprotein I (anti-beta(2)GPI) antibodies are associated with lupus nephritis (group II patients), and whether there are differences in the prevalence of these two autoantibodies between group II patients and patients with non-nephritis SLE (group I) and primary antiphospholipid syndrome (PAPS) patients (group III). MET...
متن کاملAnticardiolipin antibodies from patients with the antiphospholipid antibody syndrome recognize epitopes in both beta(2)-glycoprotein 1 and oxidized low-density lipoprotein.
BACKGROUND We recently suggested that many anticardiolipin antibodies bind only to oxidized cardiolipin (OxCL) and/or to OxCL-beta(2)-glycoprotein 1 (beta(2)GP1) adducts but not to a "reduced" cardiolipin that is unable to undergo oxidation. To test this hypothesis, we investigated 24 sera, 4 protein A-purified IgG fractions, and 3 human monoclonal antibodies that were all isolated from patient...
متن کاملChemical Modification of Activated Carbon and Its Application for Solid Phase Extraction of Copper(II) and Iron(III) Ions
Powder activated carbon surface (AC) was grinded and modified and altered procedure thorough a facile and easy chemical reaction to appearance of 2-((3silylpropylimino)1-methyl) phenol (AC- (SPIMP)). Subsequently, this novel sorbent efficiently applied for the extraction and preconcentration of some metal ions from real samples. Preliminary the influences of variables such as pH, amounts of rea...
متن کاملAnticardiolipin and anti-ß2-glycoprotein 1 in Omani patients with anti-phospholipid syndrome
Studies have shown that plasma glycoprotein with phospholipid-binding property is required for ACA., to bind to cardiolipin that has been coated onto plastic plates. This cofactor has been identified as β2-glycoprotein 1 (β2GPl). Later, it was found that β2GPl is indeed the antigen to which many ACA patients are actually binding and the phospholipid merely serves to link the β2GPl to the solid ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 179 شماره
صفحات -
تاریخ انتشار 1994